Present situation of the autochthonous donkey population in Spain

A study of the present census and administrative situation has been studied also the possibilities of conservation has been analysed During the last 5 years the donkey census has increased because the consideration like a breed in danger of extinction. There are several initiatives looking for the consolidation of the autochthonous breeds of donkeys in Spain but most of them are less important and very isolated. Except for these conservation initiatives, the donkey breeds be considered in a serious danger of extinction.Se realiza un estudio de la situación actual censal y administrativa de las razas asnales españolas y se analizan sus posibilidades y perspectivas de conservación Queda reflejado cómo en los últimos 5 años se ha producido un aumento en los efectivos asnales como respuesta a las diferentes acciones de sensibilización con respecto a las razas en peligro de extinción. No faltan iniciativas que adaptándose a los nuevos tipos de demanda busquen soluciones para la consolidación de nuestras razas autóctonas asnales aunque aún muchas de ellas son discretas y localizadas. Fuera del ámbito esas actuaciones el panorama no se muestra muy alentador

Integration and segregation across large-scale intrinsic brain networks as a marker of sustained attention and task-unrelated thought

Sustained attention is a fundamental cognitive process that can be decoupled from distinct external events, and instead emerges from ongoing intrinsic large-scale network interdependencies fluctuating over seconds to minutes. Lapses of sustained attention are commonly associated with the subjective experience of mind wandering and task-unrelated thoughts. Little is known about how fluctuations in information processing underpin sustained attention, nor how mind wandering undermines this information processing. To overcome this, we used fMRI to investigate brain activity during subjects’ performance (n=29) of a cognitive task that was optimized to detect and isolate continuous fluctuations in both sustained attention (via motor responses) and task-unrelated thought (via subjective reports). We then investigated sustained attention with respect to global attributes of communication throughout the functional architecture, i.e., by the segregation and integration of information processing across large scale-networks. Further, we determined how task-unrelated thoughts related to these global information processing markers of sustained attention. The results show that optimal states of sustained attention favor both enhanced segregation and reduced integration of information processing in several task-related large-scale cortical systems with concurrent reduced segregation and enhanced integration in the auditory and sensorimotor systems. Higher degree of mind wandering was associated with losses of the favored segregation and integration of specific subsystems in our sustained attention model. Taken together, we demonstrate that intrinsic ongoing neural fluctuations are characterized by two converging communication modes throughout the global functional architecture, which give rise to optimal and suboptimal attention states. We discuss how these results might potentially serve as neural markers for clinically abnormal attention

The preburning condition of Chalcolithic cremated human remains from the Perdigoes enclosures (Portugal)

The Iberian Chalcolithic displayed a remarkable variety of funerary practices, which has been related to interpopulation differences, intrapopulation social-cultural differences, and complex multistage funerary rituals. Perdigoes, a Chalcolithic set of ditched enclosures, reflects such diversity including a wide array of funerary practices. Among those practices is cremation, which, despite relatively rare, is represented in different structures in Perdigoes. One of these structures (Pit 40) presents an unparalleled high minimum number of individuals (n = 240), contrasting with nearby and coeval structures. In this study, we analyse heat-induced bone changes and other archaeothanatological variables to tentatively assess the preburning condition of the human remains. The results of Pit 40 are also compared with other comparable contexts to assess if this unique context presents further funerary differences relative to those other contexts in, for example, body processing. Our results suggest preferential cremation of fleshed human remains, but burning of at least a minority of skeletonised remains and deposition of possibly unburned remains also likely occurred. Body processing appears to be comparable with that of the cremation contexts of Perdigoes but contrasts with that of another nearby context (Dolmen of Olival da Pega 2b) in which burned bones were also found.Portuguese Foundation for Science and TechnologyPortuguese Foundation for Science and Technology [PEst-OE/SADG/UI0283/2013, POCI-01-0145-FEDER-016766, PTDC/EPH-ARQ/0798/2014, PTDC/IVC-ANT/1201/2014, SFRH/BPD/84268/2012]info:eu-repo/semantics/publishedVersio

Genetic inbreeding depression load for fertility traits in Pura Raza Española mares

Fertility is a key factor in the economic success of horse farms. However, it has received little attention due to the difficulty of measuring fertility objectively. Since its studbook creation (1912), the Pura Raza Española (PRE) breed has been a closed population and become high in-bred resulting in inbreeding depression (poor phenotypic values). Nevertheless, heterogeneous effects of inbreeding depression have been detected among founders and nonfounders. The aims of this study were (1) to analyze the genetic parameters for reproductive traits in mares of the PRE horse breed and (2) to estimate, for the first time, the inbreeding depression load associated with common ancestors of the breed. A total of 22, 799 mares were analyzed. Heritability estimates ranged from 0.05 (interval between first and second foaling) to 0.16 (age at first foaling), whereas inbreeding depression load ratios ranged from 0.06 (parturition efficiency at 6th foaling) to 0.17 (age at first foaling), for a partial inbreeding coefficient of 10%. Although heritability is related to the variability expressed in the population, inbreeding depression load ratios measure the potential variability, whether expressed in the population or not. Most correlations between additive and inbreeding depression load genetic values were significant (P < 0.001) and of low to moderate magnitude. Our results confirm that individual inbreeding depression loads allow us to select horses that have a genetic value resistant to the deleterious effects of inbreeding. © The Author(s) 2021. Published by Oxford University Press on behalf of the American Society of Animal Science

A synthetic-lethality RNAi screen reveals an ERK-mTOR co-targeting pro-apoptotic switch in PIK3CA+ oral cancers.

mTOR inhibition has emerged as a promising strategy for head and neck squamous cell carcinomas (HNSCC) treatment. However, most targeted therapies ultimately develop resistance due to the activation of adaptive survival signaling mechanisms limiting the activity of targeted agents. Thus, co-targeting key adaptive mechanisms may enable more effective cancer cell killing. Here, we performed a synthetic lethality screen using shRNA libraries to identify druggable candidates for combinatorial signal inhibition. We found that the ERK pathway was the most highly represented. Combination of rapamycin with trametinib, a MEK1/2 inhibitor, demonstrated strong synergism in HNSCC-derived cells in vitro and in vivo, including HNSCC cells expressing the HRAS and PIK3CA oncogenes. Interestingly, cleaved caspase-3 was potently induced by the combination therapy in PIK3CA+ cells in vitro and tumor xenografts. Moreover, ectopic expression of PIK3CA mutations into PIK3CA- HNSCC cells sensitized them to the pro-apoptotic activity of the combination therapy. These findings indicate that co-targeting the mTOR/ERK pathways may provide a suitable precision strategy for HNSCC treatment. Moreover, PIK3CA+ HNSCC are particularly prone to undergo apoptosis after mTOR and ERK inhibition, thereby providing a potential biomarker of predictive value for the selection of patients that may benefit from this combination therapy

Four years tracking unrevealed topological changes in the african interdomain

Despite extensive studies on the Internet topology, little is still known about the AS level topology of the African Internet, especially when it comes to its IXP substrate. The main reason for this is the lack of vantage points that are needed to obtain the proper information. From 2013 to 2016, we enhanced the RIPE Atlas measurement infrastructure in the region to shed light on both IPv4 and IPv6 topologies interconnecting local ISPs. We increased the number of vantage points in Africa by 278.3% and carried out measurements between them at random periods. To infer results that depict the behavior of ISPs in the region, we propose reproducible traceroute data analysis techniques suitable for the treatment of any set of similar measurements. We first reveal a large variety of ISP transit habits and their dependence on socio-economic factors. We then compare QoS within African countries, European countries, and the US to find that West African networks in particular need to promote investments in fiber networks and to implement traffic engineering techniques. Our results indicate the remaining dominance of ISPs based outside Africa for the provision of intra-continental paths, but also shed light on traffic localization efforts. We map, in our traceroute data, 62.2% of the IXPs in Africa and infer their respective peers. Finally, we highlight the launch of new IXPs and quantify their impacts on end-to-end connectivity. The study clearly demonstrates that to better assess interdomain routing in a continent, it is necessary to perform measurements from a diversified range of vantage points.The work done by Rodérick Fanou was funded by IMDEA Networks Institute as part of the project “Mapping and Measuring the African Internet”. Francisco Valera has been partially funded by the European Commission under FP7 project LEONE (FP7-317647).Publicad

Genetic parameters of biokinematic variables of the trot in Spanish Purebred horses under experimental treadmill conditions

The purpose of this study was to estimate the genetic parameters of biokinematic variables in Spanish Purebred (SPB) horses in order to select those of sufficient interest to be measured in the pre-selection of the animals for possible inclusion in the breeding programme. Kinematic analysis of 130 SPB horses 4.6 ± 1.5 years old were recorded at the trot (4 m/s) on a treadmill. Genetic parameters were estimated using VCE software and a bivariate mixed animal model including age and stud as fixed effects and animal additive genetic effect and residual error as random effects. In general, heritabilities were high (0.33–0.88). The angular variables presented the lowest heritabilities, whereas the maximum height of the fore-hoof and the duration of swing phase in the hindlimb gave the highest scores. Genetic correlations were also very high, so it was possible to reduce the number of breeding programme characteristics to stride duration, hindlimb swing phase duration, range of stifle and elbow angles, minimal angle of carpus, and minimal retraction-protraction angle of the hindlimb.Unión Europea 1FD97-089

Effect of Greenhouse Film Cover on the Development of Fungal Diseases on Tomato (Solanum lycopersicum L.) and Pepper (Capsicum annuum L.) in a Mediterranean Protected Crop

Greenhouses on the Mediterranean coast mainly use plastic materials as their cover. The influence of light exerted by these materials directly affects the crops by modifying the environment in which they develop. The aim of this study was to analyze the effect of the use of two plastic films in an experimental greenhouse on the development of fungal diseases in two spring–summer crop cycles: tomato (Solanum lycopersicum L.) from february to July 2021 and pepper (Capsicum annuum L.) from February to July 2022. The study was carried out in Almeria (Spain) in a multispan greenhouse divided transversely into two sectors by a polyethylene sheet. A commercial film was installed in the east sector (90% of transmissivity and 55% diffusivity) and an experimental film was installed in the west sector (85% of transmissivity and 60% diffusivity). In addition, the effect of the yield and quality of the harvested fruit was determined. In this study, two diseases were established naturally on the crop: (i) powdery mildew (Leveillula taurica) in both the tomato and the pepper crop cycles and (ii) early blight (Alternaria solani) in the tomato. The analyses of both diseases showed that the areas of the greenhouse that used the plastic cover, which presented a lower sunlight transmissivity, showed higher levels of disease than the areas that used the plastic cover that allowed greater transmissivity of light within the greenhouse, differing statistically in some phases of the crop. The marketable yield was 4.2% (for tomato) and 3.1% (for pepper) higher in the sector with the experimental film with high transmissivity. For both crops, the quality of the fruits did not show statistically significant difference

Elastic scattering of O-17+Pb-208 at energies near the Coulomb barrier

Within the frame of the commissioning of a new experimental apparatus EXPADES we undertook the measurement of the elastic scattering angular distribution for the system O-17+Pb-208 at energy around the Coulomb barrier. The reaction dynamics induced by loosely bound Radioactive Ion Beams is currently being extensively studied [4]. In particular the study of the elastic scattering process allows to obtain direct information on the total reaction cross section of the exotic nuclei. In order to understand the effect of the low binding energy on the reaction mechanism it is important to compare radioactive weakly bound nuclei with stable strongly-bound nuclei. In this framework the study of the O-17+Pb-208 elastic scattering can be considered to be complementary to a previous measurement of the total reaction cross section for the system F-17+Pb-208 at energies of 86, 90.4 MeV [5, 6]. The data will be compared with those obtained for the neighboring systems O-16,O-18+Pb-208 and others available in literature